Another device that fits into the eyepiece tube that can be used for precise measurements is a filar micrometer. Stereo microscopes tend to have larger diameter tubes. Before ordering an eyepiece reticle, measure the inner diameter of your microscope eyepiece. Most microscope dealers offer both the micrometer slides and the reticles. When you are finished calibrating the scope I recommend you write or print the results and tape the paper to your microscope or nearby it for quick reference. On a zoom stereoscope choose specific zoom settings (magnifications) to calibrate. The scale on the microscope slide can be used to determine the field of view in your microscope and to calibrate the ocular scale for each objective. some can measure angles, others have grids and some only have a cross on them. Reticles come in different diameters and different markings e.g. The most common reticles have a horizontal scale some reticles have both horizontal and vertical markings. Photo above shows an eyepiece reticule with a scale etched on its surface. For stereo microscopes you can use a ruler with a millimeter scale on it.įig. These microscope slides are available from microscope manufacturers including Motic for a reasonable cost. A micron or micrometer is 0.001 mm or 1/1000th of a millimeter. You need a microscope slide with a precision scale on it – usually 1 mm scale divided into 0.010 divisions. Materials needed to calibrate a Light microscope or Stereo microscope. In this article, I describe methods to calibrate your light microscope or stereo microscope to measure objects so that you can add scale bars to the images as shown above. Some of these programs have limited ability to customize the bar, fonts, color or the position of the scale bar. There is software that permits you to calibrate each objective and then it will automatically add scale bars to the picture (e.g. Once your microscope is calibrated you can add scale bars to photographs with an image editing program like Photoshop. Measuring objects with a light microscope or even a stereo microscope is straight forward, but you will need a few accessories and you will need to calibrate your microscope. Most light microscopes are used to measure organisms such as their length and width to aid in identification. Light microscopes capable of super resolution can detect objects to about 20 nm. Light microscopes can magnify specimens about 1000X and resolve objects down to 0.2 microns (200 nm, nm = 0.000001 mm).
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